Comprehensive analysis of circRNAs from cashmere goat skin by next generation RNA sequencing (RNA-seq)

RNA Sequencing with Next-Generation Sequencing

Sequencing of RNA has been an important application of DNA sequencing technology since its invention. RNA is usually sequenced by first converting it to complementary DNA (cDNA) with the reverse transcriptase enzyme (RNA-dependent DNA polymerase). Reverse transcriptase was originally isolated from Rous sarcoma retrovirus and Rauscher mouse leukemia retrovirus (R-MLV) by Baltimore (1970) and ind...

Exploring Differentially Expressed Genes by RNA-Seq in Cashmere Goat (Capra hircus) Skin during Hair Follicle Development and Cycling

Cashmere goat (Capra hircus) hair follicle development and cycling can be divided into three stages: anagen, catagen and telogen. To elucidate the genes involved in hair follicle development and cycling in cashmere goats, transcriptome profiling of skin was carried out by analysing samples from three hair follicle developmental stages using RNA-Seq. The RNA-Seq analysis generated 8487344, 81425...

Comprehensive Analysis of RNA-Seq Data

Transcriptome sequencing: next generation approach to RNA functional analysis

The most general definition of transcriptome assumes that it is a complete set of RNA molecules (transcripts) that are formed in a given organism. In response to exogenous and endogenous stimuli, transcriptome undergoes constant qualitative and quantitative changes. Monitoring these changes can provide information on how the living organisms function and deal with the changing environmental con...

Preparation of Single-Cell RNA-Seq Libraries for Next Generation Sequencing.

For the past several decades, due to technical limitations, the field of transcriptomics has focused on population-level measurements that can mask significant differences between individual cells. With the advent of single-cell RNA-Seq, it is now possible to profile the responses of individual cells at unprecedented depth and thereby uncover, transcriptome-wide, the heterogeneity that exists w...